Table 2. Crystallization.
Method | Hanging-drop vapor diffusion |
Plate type | 24-well Linbro plate |
Temperature (K) | 298 |
Protein concentration (mg ml−1) | 10 |
Buffer composition of protein solution | 20 mM Tris pH 8.0, 500 mM NaCl, 0.5 mM TCEP, 1 mM MnCl2 |
Composition of reservoir solution | 0.1 M HEPES pH 7.8, 0.2 M ammonium sulfate, 0.01 M cadmium chloride hemi(pentahydrate), 25%(v/v) PEG Smear Medium |
Volume and ratio of drop | 3 µl; 1:2 condition:protein |
Volume of reservoir (ml) | 0.5 |