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. 2018 Nov 30;74(Pt 12):817–824. doi: 10.1107/S2053230X18016503

Table 2. Crystallization.

Method Hanging-drop vapor diffusion
Plate type 24-well Linbro plate
Temperature (K) 298
Protein concentration (mg ml−1) 10 
Buffer composition of protein solution 20 mM Tris pH 8.0, 500 mM NaCl, 0.5 mM TCEP, 1 mM MnCl2
Composition of reservoir solution 0.1 M HEPES pH 7.8, 0.2 M ammonium sulfate, 0.01 M cadmium chloride hemi(pentahydrate), 25%(v/v) PEG Smear Medium
Volume and ratio of drop 3 µl; 1:2 condition:protein
Volume of reservoir (ml) 0.5