Fig. 2.

Confocal Laser Scanning Microscopy (CLSM) images of C. albicans biofilms formed from the CYR1OE and PDE2OE strains. A: a1–4: Farnesol untreated wild strain. b1–4: Farnesol untreated CYR1OE strain. c1–4: Farnesol untreated PDE2OE strain. d1–4: Farnesol treated wild strain. e1–4: Farnesol treated CYR1OE strain. f1–4: Farnesol treated PDE2OE strain. Scale bars represent 20 μm for 400× magnifications. The biofilms of d1–4, e1–4 and f1–4 consisted of fewer hyphae, but more pseudohyphae and yeast than did those of a1–4, b1–4 and c1–4. Additionally, the biofilms of b1–4 and c1–4 consisted of more hyphae and pseudohyphae than did that of a1–4. B: The biofilms exposed to farnesol were thinner than that without farnesol and the biofilms of the CYR1OE and PDE2OE strains were much thicker than that of the wild strain. Significance was calculated using one-way ANOVA with post ad-hoc Dunnett’s multiple comparison test. *: p < 0.05. **: p < 0.01