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. 2018 Dec 3;25:89. doi: 10.1186/s12929-018-0488-3

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — A cartoon depicting an example of cell seeding using the hanging drop method within the well of a specialized hanging drop plate that uses surface tension and gravity to form spheroids. The cells come together at the air-liquid interface and form a single spheroid. (Reprinted with permission from [91])