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. 2018 Dec;59(15):5795–5802. doi: 10.1167/iovs.18-25215

Figure 1.

Figure 1

(A) Average percentile rank of common endothelial cell-enriched genes. 1.00 refers to genes expressed approaching the 100th percentile compared to all mapped genes in the sample. (B) PECAM1 mRNA abundance by qPCR using two independent primer sets that amplify homologous regions of human and rhesus mRNAs. Normalized to B2M. (C) Immunoblotting for PECAM1 in HUVEC and RF/6A. Low (left) and high (right) exposures shown. (D) Immunohistochemistry of M. fascicularis eye with the JC/70A PECAM1 antibody. Reaction product is blue. (E) Abundance of CDH5 mRNA, which encodes for VE-Cadherin (VE-Cad), by qPCR using primers that amplify a homologous region of human and rhesus mRNAs. Normalized to B2M. (F) Immunoblotting of VE-Cad in HUVEC and RF/6A. Low (left) and high (right) exposures shown. (G) VE-Cad immunoblotting of HUVEC, RF/6A-1, and mREC. INL, inner nuclear layer; OPL, outer plexiform layer; RPE, retinal pigmented epithelium; CC, choriocapillaris; Sattler, Sattler's layer; Haller, Haller's layer.