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. 2018 Oct 24;23(11):2750. doi: 10.3390/molecules23112750

Figure 3.

Figure 3

PP2A activity of compound 7 in HT-29 cells. (A) PP2A activity was measured in HT-29 cells treated with the 20 μM of compound 7 and compound 1 (FTY720) for 12 h. (B,C) Cells were treated with or without 20 μM compound 7 and FTY720 for 12 h, protein extracted, separated, and probed with pERK1/2, ERK1/2, pAKT and AKT. The band was analyzed by UVITEC imaging software, and data were plotted as means ± SD for three replicates of the experiments. * p < 0.05, ** p < 0.01 compared with control cells.