Figure 3.

Pinnatane A caused cell cycle arrest in Hep3B and HepG2 cell lines. (A) Cell cycle distribution of Hep3B and HepG2 cells with pinnatane A treatment for various time points (12, 24, and 48 h) using flow cytometry after staining with PI. (B) Hep3B and (C) HepG2 cells cell cycle phase distribution presented in four groups, which are the group I: sub-G₀/G₁ phase, group II: G₀/G₁ phase, group III: S phase, and group IV: G₂/M phase. All results are expressed in the histogram as total percentages of cells from four different groups with mean ± SD of three independent determinations. All data collected from experiments were performed in three replicates and analyzed using the one-way analysis of variance (ANOVA) at a significance level of p < 0.05 and indicated by *.