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. 2018 Nov 16;23(11):2993. doi: 10.3390/molecules23112993

Figure 2.

Figure 2

Effect of the BSA/SMNPs mass ratio on the immobilization parameters (RA—recovered activity, IY—immobilization yield, and GY—global yield) of porcine pancreas lipase (PPL) as CLEAs. Assay conditions: precipitation with ethanol (enzyme solution/ethanol volume ratio of 1:3), co-aggregation with additives (enzyme/total additives mass ratio of 1:3), crosslinking with glutaraldehyde (5 µmoles of glutaraldehyde/mg total protein), temperature of 4 °C, 30 min precipitation/aggregation and 2.5 h crosslinking under 150 rpm stirring.