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. 2018 Oct 9;41(1):27–42. doi: 10.3892/or.2018.6780

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Copyright: © Zhao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 8. — As₂S₂ induces apoptosis in breast cancer cells. (A) MCF-7 and (B) MDA-MB-231 cells were treated with different concentrations of As₂S₂ (0, 4, 8 and 16 µM) for 48 h, followed by staining with Annexin V/PI, and then analyzed by flow cytometry. The cells were assessed for the total number of apoptotic cells, including early-apoptotic (Annexin V⁺/PI⁻) and late-apoptotic (Annexin V⁺/PI⁺) cells. Results are expressed as the mean ± standard error of the mean (n≥3). *P<0.05, **P<0.01 vs. control (0 µM As₂S₂). PI, propidium iodide; PE, phycoerythrin.

Figure 8. — As₂S₂ induces apoptosis in breast cancer cells. (A) MCF-7 and (B) MDA-MB-231 cells were treated with different concentrations of As₂S₂ (0, 4, 8 and 16 µM) for 48 h, followed by staining with Annexin V/PI, and then analyzed by flow cytometry. The cells were assessed for the total number of apoptotic cells, including early-apoptotic (Annexin V⁺/PI⁻) and late-apoptotic (Annexin V⁺/PI⁺) cells. Results are expressed as the mean ± standard error of the mean (n≥3). *P<0.05, **P<0.01 vs. control (0 µM As₂S₂). PI, propidium iodide; PE, phycoerythrin.