Table 1.
Observable/Experimental physicochemical properties of selected dyes and quenchers.
| Molecular Weight | Rotatable Bonds | H-Bond Acceptors | Molar Absorptivity (M−1 cm−1) | Quantum Yield (%) | |
|---|---|---|---|---|---|
| DYES | |||||
| ICG | 751.97 | 10 | 6 | 183,000 | 3 |
| MB | 284.40 | 0 | 1 | 95,000 | 3 |
| Evans Blue | 868.85 | 4 | 20 | 7,810 | N/A |
| IR780 iodide | 540.20 | 4 | 0 | 274,000 | 7.6 |
| IRDye800CW | 1097.23 | 18 | 15 | 181,458 | 3.4 |
| Rhodamine 800 | 396.50 | 0 | 2 | 113,302 | 25 |
| Coomassie Blue | 802.98 | 11 | 7 | 43,000 | N/A |
| QUENCHERS | |||||
| IRDyeQC-1 | 1188.82 | 23 | 15 | 96,000 | N/A |
| BHQ3 | 535.66 | 7 | 7 | 42,700 | N/A |
| BlackBerry Quencher 650 | 576.60 | 6 | 12 | 41,000 | N/A |
| QSY21 | 779.88 | 4 | 5 | 90,000 | N/A |