Table 3.
Identification of small–molecule saccharides by HPLC–QTOF–MS/MS technology
| No | tR (min) | [M − H]− (m/z) (Δppm) | Fragment Ions (m/z) | Molecular Formula | Compound |
|---|---|---|---|---|---|
| 1 | 7.09 | 323.1003 (−4.98) | 323.0990; 179.0564; 161.0461; 143.0364; 113.0258; 101.0251 | C12H20O10 | Hexose derivative |
| 2 | 8.16 | 323.1010 (−6.13) | 323.1011; 179.0567; 161.0464; 143.0386; 113.0230; 101.0267 | C12H20O10 | Hexose derivative |
| 3 | 9.12 | 179.0581 (−0.58) | 179.0533; 161.0458; 143.0354; 113.0242; 101.0245 | C6H12O6 | Fructose |
| 4 | 10.43 | 181.0739 (−0.36) | 181.0715; 163.0629; 149.0436; 119.0338; 101.0247 | C6H14O6 | Sorbitol |
| 5 a | 11.60 | 179.0566 (−2.94) | 179.0583; 161.0433; 143.0350; 113.0248; 101.0248 | C6H12O6 | Glucose |
| 6 a | 11.60 | 179.0585 (−0.08) | 179.0574; 161.0480; 143.0336; 112.9853; 101.0246 | C6H12O6 | Galactose |
| 7 | 14.4 | 179.0587 (−0.65) | 179.0583; 161.0451; 143.0378; 112.9846; 101.0241 | C6H12O6 | Hexose |
| 8 | 15.24 | 341.1125 (−1.14) | 341.1090; 179.0555; 161.0465; 143.0338; 119.0350; 101.0243 | C12H22O11 | Sucrose |
| 9 | 23.71 | 503.1666 (−1.1) | 503.1668; 341.1101; 323.0988; 221.0670; 179.0554; 161.0441; 143.0328; 113.0220; 101.0240 | C18H32O16 | Trisaccharide |
| 10 | 26.49 | 503.1670 (−4.19) | 503.1668; 341.1054; 323.0986; 221.0655; 179.0553; 161.0467; 143.0368; 113.0255; 101.0244 | C18H32O16 | 1-Kestose |
| 11 | 33.15 | 503.1653 (2.62) | 503.1653; 341.1031; 323.0936; 221.0616; 179.0513; 161.0412; 143.0308; 113.0216; 101.0219 | C18H32O16 | Raffinose |
a Characterization with sole standard glucose or galactose.