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Heparanase assay using HTRF®. HSPG labelled with Eu-cryptate and biotin is detected by measuring time-resolved fluorescence after the addition of XL665-labeled streptavidin (XL665-SA). Degradation of substrate by heparanase decreases the HTRF signal. B, biotin; SA, streptavidin; FRET, fluorescence resonance energy transfer. Reproduced with permission [38].
