Table 2.
IC50 and Ki values of the isolated hop-compounds for the respective reductases. IC50 and Ki values are presented as mean ± SD of at least three experiments. (n. d. = not determined).
| Enzyme | AKR1A1 | AKR1B1 | AKR1B10 | |||
|---|---|---|---|---|---|---|
| Substrate | Glyceraldehyde [3.6 mM] | Glyceraldehyde [50 µM] | Glyceraldehyde [4.0 mM] | |||
| Parameter | IC50 | Ki (Morrison) | IC50 | Ki (Morrison) | IC50 | Ki (Morrison) |
| Compound 1 | ≥100 µM | n. d. | >125 µM | n. d. | 5.41 ± 0.42 µM | 3.27 ± 0.52 µM |
| Compound 2 | >100 µM | n. d. | >125 µM | n. d. | 1.35 ± 0.07 µM | 0.70 ± 0.09 µM |
| Compound 3 | >100 µM | n. d. | >125 µM | n. d. | 1.94 ± 0.10 µM | 0.98 ± 0.12 µM |