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. 2018 Oct 12;41(1):178–190. doi: 10.3892/or.2018.6790

Figure 2.

Figure 2.

Effect of hypoxia on HIF-1α immunoreactivity in DAOY cells. Cells were incubated under normoxia (16.2% O2) or hypoxia (1 or 0.1% O2) for 24 h, and then stained with an antibody against HIF-1α. Positive cells (A) and cells with nuclear immunoreactivity (B) were quantified in three independent experiments. **P<0.01, ***P<0.001; NS, no significant difference; one-way ANOVA and Tukey's test.