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Cellular and nuclear HIF-1α immunoreactivity in response to hypoxia in DAOY cells. After incubation for 24 h in normoxia (16.2% O2) or hypoxia (1 or 0.1% O2) DAOY cells were stained with an antibody against HIF-1α. Images are representative of three independent experiments. The quantitative analysis is shown in Fig. 2.
