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Effect of hypoxia on CYP isoform expression. DAOY cells were incubated for 24 h under normoxia (16.2% O2) or hypoxia (1 or 0.1% O2). The expression of CYP2B6 (A), CYP3A4 (B) and CYP3A5 (C) was determined by immunoblotting. Representative blots are shown. For graphs, values are means ± SEM of the relative optical density (R.O.D.) from four independent experiments. *P<0.05; NS, no significant difference; one-way ANOVA and Tukey's test.
