Figure 8.
Effect of 2-methoxyestradiol (2-ME) on hypoxia-induced downregulation of the expression of CYP isoforms. Cells were incubated under normoxia (16.2% O2) or hypoxia (1 or 0.1% O2) for 24 h and the expression of CYP2B6 (A), CPY3A4 (B) and CPY3A5 (C) was determined by immunoblotting. Representative immunoblots are depicted. The companion graphs represent the means ± SEM of the relative optical density (R.O.D.) from three independent experiments. *P<0.05, **P<0.01, ***P<0.001 vs. values in normoxia and without 2-ME; NS, no significant difference; two-way ANOVA and Bonferroni's test.