Table IV.

Apoptosis induced by CPA or IFA alone or in combination with 2-ME.

	Annexin V+/IP+ cells (%)
	16.2% O2	1% O2	0.1% O2
Control	5.5±1.1	11.0±2.1	6.1±2.5
2-ME	9.7±3.8a	19.4±5.7a	13.9±2.1a
CPA	22.5±2.8	62.9±1.4	38.2±4.7
CPA+2-ME	27.6±4.7b	78.8±2.4b	68.9±6.6d
IFA	24.0±4.4	67.8±1.1	42.0±1.7
IFA+2-ME	22.9±4.5	86.6±2.7c	75.9±2.6e

Cells were incubated for 24 h in normoxia (16.2% O₂) or hypoxia (1 or 0.1% O₂) with CPA (18.6 mM) or IFA (14.7 mM) in the absence or the presence of 2-ME (5 µM), and apoptosis was evaluated with Annexin V-FITC/PI staining and flow cytometry. Values are means ± SEM from three independent experiments.

^aP<0.05 vs. control values

^bP<0.05 vs. CPA alone

^cP<0.01 vs. IFA alone

^dP<0.001 vs. CPA alone

^eP<0.001 vs. IFA alone. The statistical analysis was performed with one-way ANOVA and Tukey's test. PI, propidium iodide; 2-ME, 2-methoxyestradiol; CPA, cyclophosphamide; IFA, ifosfamide.