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. 2018 Oct 11;41(1):333–340. doi: 10.3892/or.2018.6784

Figure 5.

Figure 5.

Knockdown of the expression of Matr3 results in induction of oral squamous cell carcinoma cell apoptosis. HSC2 and HSC3 cells were transfected with siCon or siMatr3. (A) mRNA expression level of Matr3 in each cell transfected with siCon or siMatr3 was analyzed by reverse transcription-polymerase chain reaction analysis. β-actin was used as a loading control. (B) siCon- or siMatr3-transfected HSC2 and HSC3 cell lysates were determined by western blot analysis using anti-Matr3, anti-caspase-3, anti-cleaved caspase-3, anti-PARP and anti-cleaved PARP antibodies. GAPDH was used as the loading control. (C) HSC2 and HSC3 cells were transfected with siCon or siMatr3 and soft agar assays were performed. Colony numbers and sizes were measured, and the results are expressed as the mean ± standard deviation (*P<0.05) for triplicate experiments. siMatr3, matrin 3-specific targeting siRNA; siCon, scrambled control siRNA; PARP, poly (ADP-ribose) polymerase.