Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Oct 22;41(1):165–177. doi: 10.3892/or.2018.6811

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Figure 3. — LV-siRGS5 effectively inhibits the expression of RGS5 at the mRNA and protein level in ODMECs. (A) At the mRNA level, the blank group, the negative control group LV-H-siRNA and the LV-siRGS5 three groups of quantitative analysis were 1.07±0.05, 1.32±0.09 and 0.52±0.06, 0.60±0.04, 0.54±0.02, respectively; the expression of negative control LV-H did not affect the RGS5 mRNA (n=3, P<0.05). (B) At the protein level, the blank group, the negative control group LV-H-siRNA and the LV-siRGS5 three groups of quantitative analysis was 0.92±0.08, 0.95±0.21 and 0.17±0.04, 0.22±0.08, 0.19±0.12, respectively; the negative control LV-H-siRNA did not affect the expression of RGS5 (n=3, P<0.05). (C) LV-siRGS5 did not interfere with the expression of RGS2, RGS4 and RGS16 in the B/R4 subfamily. RGS5, regulator of G-protein signaling 5; ODMECs, ovarian carcinoma-derived endothelial cells; LV, lentiviral vector.