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. 2018 Nov 27;9:2949. doi: 10.3389/fmicb.2018.02949

FIGURE 2.

FIGURE 2

Lithium chloride suppresses HCV replication. (A) Immunoblot analysis was carried out for phosphorylated-GSK3α/β (Ser 9/21) after stimulation of Huh-7.5 cells harboring Con1 replicon with 25 mM LiCl for 2 h. β-actin was detected as a loading control. (B) Suppression of subgenomic HCV replication by LiCl. Huh-7.5 cells harboring Con1 replicon were stimulated with LiCl for 24 or 48 h at the indicated concentrations. HCV RNA levels normalized to GAPDH mRNA are expressed relative to untreated cells. (C) Assessment of lithium chloride-induced cytotoxicity. WST-1 cytotoxicity assay was carried out for cells stimulated for 24 and 48 h with LiCl at the indicated concentrations. Absorbance readings (A450 – A690 nm) of treated cells were compared to negative control cells treated with the dimethyl sulfoxide vehicle. Data are presented as mean ± SEM of six independent experiments. (∗∗P-value ≤ 0.005; ∗∗∗, P-value ≤ 0.0005).