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. 2018 Nov 27;9:2949. doi: 10.3389/fmicb.2018.02949

FIGURE 3.

FIGURE 3

GSK3β inhibition suppresses full-length HCV replication as well as production of infectious HCV particles. (A) Inhibition of infectious HCV clone Jc1 replication by GSK3 inhibition. Huh-7.5 cells electroporated with HCV Jc1 full-length RNA were treated with 5 μM CHIR99021 for 72 h post-transfection. HCV RNA levels normalized to GAPDH mRNA are expressed relative to untreated cells. Data are presented as mean ± SEM of two experiments performed in triplicate. f.c., fold change. (B) Suppression of HCV infectious particles production by GSK3 inhibition. Virus infectivity was determined by foci forming assay in supernatants from Huh-7.5 cells electroporated with HCV Jc1 RNA and cultured 48 h in the presence of 5 μM CHIR99021. Results were shown as infectivity level normalized to control samples cultured in presence of 0.1% dimethyl sulfoxide vehicle. Asterisks denote statistically significant differences (∗∗P-value ≤ 0.005; ∗∗∗P-value ≤ 0.0005).