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. Author manuscript; available in PMC: 2018 Dec 4.
Published in final edited form as: Dev Biol. 2018 Apr 19;440(1):1–12. doi: 10.1016/j.ydbio.2018.04.014

Fig. 6.

Fig. 6.

ns3 pros double knockdown shows intermediate phenotype between ns3 or pros single knockdown. Single slice confocal images of single or double knockout L3 brains; NBs are identified by Dpn and NBs and their lineages are marked with GFP (wor-gal4, UAS-mCD8:GFP). Representative brains are shown (n = 5 brains per genotype). (A) ns3 RNAi brain has fewer EdU+ cells. Genotype: UAS-Dcr-2; wor-gal4; UAS-mCD8:GFP/UAS-ns3-RNAi. (B) pros RNAi brain has many more EdU+ cells. Genotype: UAS-Dcr-2; wor-gal4/UAS-pros-RNAi; UAS-mCD8:GFP. (C) ns3 pros double RNAi brain has intermediate number of EdU+ cells. Genotype: UAS-Dcr-2; wor-gal4/UAS-pros-RNAi; UAS-mCD8:GFP/UAS-ns3-RNAi. Scale bar: 50 μm.