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. 2018 Nov 27;9:2916. doi: 10.3389/fmicb.2018.02916

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Copyright © 2018 Nagel, Bieber, Schmidt-Dannert, Nett and Peters.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IDS, CPS, and KS enzyme activity. GC-MS chromatogram from an in vitro enzyme assay of PmIDS with dimethyl allyl diphosphate (DMADP) and isopentenyl diphosphate (IDP) as substrates, with subsequent dephosphorylation with alkaline calf intestine phosphatase. GC-MS chromatograms of organic extracts from E. coli cultures expressing PmKS or synthetic sPmCPS along with either AtCPS or AtKS, with additional engineering for GGDP production. GC-MS chromatograms of geranylgeraniol (dephosphorylated GGDP) and ent-kaurene standards are included for reference.