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. 2018 Nov 27;9:2916. doi: 10.3389/fmicb.2018.02916

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Copyright © 2018 Nagel, Bieber, Schmidt-Dannert, Nett and Peters.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CYP114+Fd and CYP114 + Fd-SDR enzyme activity. GC-MS chromatograms from organic extracts of E. coli cultures expressing PmCYP114 either with the artificial PmFd or the PmFd-SDR fusion protein found in P. mimosarum and fed ent-kaurenoic acid. GC-MS chromatograms of ent-7a-hydroxykaurenoic acid, GA₁₂-aldehyde and GA₁₂ standards are included for reference.