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. 2018 Nov 27;12:103. doi: 10.3389/fncir.2018.00103

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Copyright © 2018 Omoto, Nguyen, Kandimalla, Lovick, Donlea and Hartenstein.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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R-neuron subclasses represent sublineages of DALv2. (A) Schematized overview of MARCM clone induction and resultant clone categories. Neuroblast clones result in labeling of the entire lineage from induction onward; ganglion mother cell (GMC) clones result in singly-labeled neurons. (B) Histogram of single cell clone R-neuron subclasses generated by temperature shifts during distinct time windows throughout larval development. A total of 33 single cell clones were generated, % reflect the proportion of clones of a given subclass generated during a given time window. (C–E) Representative clones (green) from three time windows after hatching (AH). Axon tracts labeled by anti-Neuroglian (gray). (C) R4m. (D) R5. (E) R3. Scale bars represent 25 μm (C–E).