Table 3.

Advantages and disadvantages of chemical ligation.

Advantages	Disadvantages	Ref.
Good yield Chemo-selective or high specificity reaction Side-chain protection groups unnecessary NCL produce peptide bond The absence of racemisation at the site ligation	In some case, the reaction typically takes 1–3 days to go to completion The additional purification step is necessary Produce peptide with no natural bond NCL requires cysteine for ligation	[22]