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. 2018 Dec 4;5:180287. doi: 10.1038/sdata.2018.287

Figure 3. Polysome profiling followed by RNA-seq during cardiomyogenic differentiation.

Figure 3

(a) Schematic representation of the sucrose gradient used to segregate ribosome-free and ribosome-bound RNAs and representative polysome profile (hESCs - D0 replicate 2) recorded at 254 nm. Ribosome-free and polysome fractions are indicated. (b-d) Representative quality analysis of ribosome-free and polysome-bound samples. (a) RNA quality analysis using Agilent 2100 Bioanalyzer. (b) cDNA library quality analysis using Agilent 2100 Bioanalyzer. (c) RNA-sequencing reads quality analysis using FastQC. All representative images correspond to D0 hESC sample, replicate 2.