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. 2018 Dec 4;5:180287. doi: 10.1038/sdata.2018.287

Table 2. Primer sequences used for qPCR analysis of cardiac developmental genes.

Gene Sequence (5’-3’) Access number Amplicon (pb) Reference
POU5F1 F: ATGCATTCAAACTGAGGTGCCTGC NM_001173531 192 pb (YE et al., 2013)
R: AACTTCACCTTCCCTCCAACCAGT      
NANOG F: ACCAGAACTGTGTTCTCTTCCACC NM_024865 200 pb (ZAEHRES et al., 2005)
R: CCATTGCTATTCTTCGGCCAGTTG      
T F: AAAGAGATGATGGAGGAACCCGGA NM_003181 108 pb (YE et al., 2013)
R: AGGATGAGGATTTGCAGGTGGACA      
EOMES F: CAAATTCCACCGCCACCAAACTGA NM_001278182.1 108 pb (OVCHINNIKOV et al., 2014)
R: TTGTAGTGGGCAGTGGGATTGAGT      
GATA4 F: ACCTGGGACTTGGAGGATAGCAAA NM_002052 169 pb (YE et al., 2013)
R: TCCCATCAGCGTGTAAAGGCATCT      
NKX2.5 F: TTAAGTCACCGTCTGTCTCCCTCA NM_001166175 124 pb (YE et al., 2013)
R: ACCGACACGTCTCACTCAGCATTT      
TNNT2 F: TGCAGGAGAAGTTCAAGCAGCAGA NM_000364 155 pb (YE et al., 2013)
R: AGCGAGGAGCAGATCTTTGGTGAA      
TNNI3 F: GGGGGCCCGGGCTAAGGAGTC NM_000363.4 183 pb (SCHITTINI et al., 2010)
R: AGGGCAGGGGCAGTAGGCAGGAAG      
GAPDH F: GGCGATGCTGGCGCTGAGTAC NM_002046.3 149 pb PrimerBlast
R: TGGTTCACACCCATGACGA