Table 4. Summary statistics of the cDNA sequencing reads – derived from the different time points - aligned to the Sf9 genome.

Sample	Number of aligned reads	Median of read lengths	Average of read lengths±SE	Average of aligned read lengths±SE	Average Insertion frequency (%)±SE (%)	Average Deletion frequency (%)±SE (%)	Average Mismatch frequency (%)±SE (%)	Coverage	H50
Read N50 is defined as the length N for which 50% of all bases in the reads are in a sequence of length L<N.
0h	169794	680	773.75±0.99	586.81±0.94	3.69±0.01	3.77±0.01	4.94±0.01	0.19	649
1h	20346	642	961.12±6.74	719.88±6.50	3.54±0.02	3.24±0.02	4.54±0.02	0.03	796
2h	27209	588	701.67±2.77	466.31±2.64	3.41±0.02	3.53±0.02	4.62±0.02	0.02	765
4h	46519	611	730.90±1.90	475.63±1.87	3.36±0.02	3.35±0.01	4.44±0.01	0.04	611
6h	60076	627	738.99±1.68	517.17±1.59	3.52±0.01	3.58±0.01	4.72±0.01	0.06	438
16h	275282	683	841.67±1.11	656.40±1.08	3.85±0.00	3.54±0.00	4.92±0.00	0.35	617
24h	299940	641	733.99±0.71	550.25±0.69	3.79±0.00	3.61±0.00	4.96±0.00	0.32	621
48h	80781	687	826.03±1.86	632.02±1.77	3.83±0.01	3.58±0.01	5.00±0.01	0.10	610
72h	118547	750	883.51±1.32	683.21±1.28	3.97±0.01	3.79±0.01	5.19±0.01	0.16	1629