Table 5. Summary table of the reagents and chemistries used for the sequencing.

Total RNA isolation	PolyA selection	Ribodepletion	Reverse transcription & dscDNA production	cDNA synthesis by PCR	Library preparation kit
Macherey-Nagel RNA	Qiagen Oligotex mRNA mini Kit	-	SuperScript III	-	Direct RNA Sequencing Kit
			SuperScript IV	KAPA HiFi PCR Kit	Ligation Sequencing Kit 1D
					PCR Barcoding Expansion 1-96+Ligation Sequencing Kit 1D
	-	Epicentre Ribo-Zero™ Magnetic Kit H/M/R	Lexogen Teloprime Kit enzymes & reagents	Lexogen Teloprime PCR mix	Ligation Sequencing Kit 1D