Figure 1.

PHD3 altered activation of the EGFR/HIF-1α pathway in Mia-paca2 cells treated with 2 Gy radiation under normoxic and hypoxic conditions.

Notes: (A and B) Immunoblotting of the indicated proteins in Mia-paca2 cells with or without PHD3 overexpression after radiation treatment (2 Gy) under normoxic or hypoxic conditions. Protein extraction from cells at 24 hours after radiation treatment was used for immunoblotting. β-actin served as loading control. (C and D) Relative protein expression of p-EGFR/EGFR and HIF-1α under normoxia or hypoxia. ^*P<0.05, ^**P<0.001, ^***P<0.0002, ^****P<0.0001, compared with non-irradiated normal cells.

Abbreviations: HIF, hypoxia-inducible factor; PHD, proline hydroxylase domain.