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. 2018 Nov 29;11:8455–8463. doi: 10.2147/OTT.S186498

Figure 5.

Figure 5

Combined treatment with GSK126 and Gefitinib significantly inhibited tumor xenograft in nude mice but did not affect body weight and liver function.

Notes: The nude mice transplanted with MGC803 cells tumor xenografts were randomly divided into four groups that received i.p. injection of GSK126 (200 mg/kg) and/or Gefitinib (30 mg/kg) for 4 weeks. (A) Tumor volume was recorded every other day. (B) Mice were sacrificed on day 28 and tumor wet weight was measured. (C) The body weight of nude mice was recorded every other day. (D) The content of AST and ALT was determined by ELISA using commercial kits. (E) Representative blots of ULK and LC3 proteins in tumor tissues. (F) Quantitative analysis of p-ULK/t-ULK and LC3-II/LC3-I. *P<0.05, **P<0.01, n=8–10 mice/group.

Abbreviations: i.p., intraperitoneal; p-ULK, phosphorylated ULK; ULK, Unc-51-like autophagy activating kinase; ALT, alanine transaminase; AST, aspartate transaminase; t-ULK, total ULK.