Fig. 7.

Effect of γ-irradiation on ampC and ampG expression of E. coli O157:H7; (a): in wild type bacteria. (b): in 25 μg/mL carbenicillin adapted bacteria (Carb25). Relative gene expression represents the change in transcription in bacteria 60 minutes after irradiation treatment at a dose of 0.4 kGy. Results are represented by log₂ RNA expression using −2^ΔΔCt. The difference was considered as significant when the expression relative variation ≥1.5 log₂. Carb25: 25 μg/mL carbenicillin adapted bacteria, NI: not irradiated and Irr: irradiated.