Figure 4.

Effect of oral leucine (Leu) gavage on muscle protein synthesis, and plasma leucine and insulin concentrations in control‐ and alcohol‐fed rats. Both male and female rats were fed an alcohol‐containing liquid diet or isocaloric isonitrogenous diet for 14 weeks, then fasted overnight, and administrated an oral gavage of a maximally stimulating dose of Leu (1.35 g/kg body weight) and gastrocnemius and blood collected 1 h thereafter. Values are means ± SEM; n = 7–8 for each treatment group for each male and female rat. Values with different letters are statistically different (P < 0.05) from each. Data were analyzed by one‐way ANOVA with Dunnett's post hoc test against the sex‐specific control value (e.g., no leucine or EtOH group).