Table 1|.
Growth rate estimation from parameters measured
| Sample | R/P ratio | rRNa frac. | ϕRcomplex | ϕRbound | kel (amino acids per second) | Estimated growth rate from all 70 S (h–1) | Estimated growth rate from bound 70 S (h–1) | Actual growth rate (h–1) |
|---|---|---|---|---|---|---|---|---|
| C-0.1 | 0.16 | 0.64 ± 0.03 | 0.83 ± 0.02 | 0.31 ± 0.03 | 12.4 ± 0.2 | 0.29 ± 0.02 | 0.09 ± 0.01 | 0.09 ± 0.00 |
| N-0.1 | 0.16 | 0.59 ± 0.06 | 0.84 ± 0.02 | 0.51 ± 0.03 | 8.1 ± 0.1 | 0.17 ± 0.01 | 0.09 ± 0.01 | 0.09 ± 0.00 |
| P-0.1 | 0.08 | 0.51 ± 0.03 | 0.81 ± 0.03 | 0.65 ± 0.04 | 12.4 ± 0.1 | 0.12 ± 0.01 | 0.08 ± 0.01 | 0.09 ± 0.01 |
Estimation of growth rate using measurements from R/P ratio, fraction of rRNA in total RNA (rRNA frac.), elongation rates (kel) and fractions of different ribosomal species (ϕRcomplex and ϕRbound). All values are mean values. R/P ratio values are derived from three technical replicates and the rest of the parameters are from three biological replicates with the s.e.m. shown. The propagated error is shown for growth rate estimates.