Figure 4.

Intracellular uptake of liposomes, 48 hours after insertion.

Notes: (A) More intracellular uptake was observed in cultures with the small liposomes (~75 nm), compared with cultures with the intermediate (~100 nm) and large liposomes (~180 nm). Intracellular liposome uptake is present both in the small and intermediate liposome plugs. Data are presented as mean ± SD. *P<0.05, **P<0.01. (B) Confocal images of intracellular liposomes in 3D, showing nuclei (DAPI, blue), f-actin (phalloidin, red), and liposomes (DiD, cyan). All cultures were washed extensively before imaging, to remove all noninternalized liposomes. The scale bars represent 200 µm.