Figure 2.
Optical Properties of MitoNeo and Its Derivatives
(A) UV-visible spectra of MitoNeo, MitoNeoOH, and MitoNeoH (25 μM of each) in ethanol.
(B–D) Fluorescence spectra of MitoNeo and/or MitoNeoOH (25 μM of each in KCl buffer). (B) Fluorescence spectra of MitoNeo and/or MitoNeoOH as a function of excitation and emission wavelength, shown in 3D (upper) and 2D (lower) views. (C) Emission fluorescence spectra of MitoNeo and MitoNeoOH. Excitation wavelengths: 566 nm (MitoNeo), 544 nm (MitoNeoOH). (D) Excitation fluorescence spectra of MitoNeo and MitoNeoOH. Emission wavelengths: 636 nm (MitoNeo), 605 nm (MitoNeoOH).
(E) Concentration dependence of fluorescence of MitoNeoOH and MitoNeo at the excitation (544 nm) and emission (605 nm) maxima of MitoNeoOH.