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. Author manuscript; available in PMC: 2018 Dec 4.
Published in final edited form as: Phys Chem Chem Phys. 2018 Oct 31;20(42):26892–26902. doi: 10.1039/c8cp04112a

Fig. 4.

Fig. 4

Comparison of dynamic versus static binding events for wild type and Pol III core mutant containing a Y453A mutation in the thumb domain of the α-subunit using the method of maximum likelihood analysis for the G:AA double mismatch. Single-molecule experiments were performed in 20 mM Hepes, pH 7.6, 5 mM potassium glutamate, 3 mM magnesium acetate, 2 mM DTT, 2 mM Trolox, 25 nM enzyme and 10 μM dCTP. (A) Example of a dynamic event (left) that has distinct changes in FRET over the course of the binding event and a static event (right) that has one FRET state through the duration of the binding event. (B) Frequency of dynamic and static binding events for wild type (n = 100) and mutant (n = 96) DNA Pol III. Here, the wild type has a higher frequency of dynamic events (49%) than the mutant (21%) as shown in blue.