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Combination blockade of the PD1, CTLA-4 and PD-L1 coinhibitory molecules coupled with Fvax vaccination increased survival of mice challenged with antigen-presenting melanoma cells. Lack of survival was defined as death or tumour size >1000 mm3. CTLA-4, cytotoxic T-lymphocyte-associated antigen-4; PD1, programmed death 1; PDL-1, programmed death ligand-1.
