Figure 5.

SK053 decreases the percentage of KG1 leukemia-initiating cells and their clonogenic potential in vitro. (A) KG1 cells were incubated for 72 h with the indicated concentrations of SK053, harvested and stained for flow cytometry. Representative density plots from a series of experiments are presented. (B) Mean percentages of CD123⁺ KG1 cells among CD34⁺ and CD38⁻ cells ± SD (n=3 experiments) ^*P<0.05 vs. controls, one-way ANOVA with Dunnett post-hoc test. (C) Representative pictures of the clonogenic assay plates on day 14 after seeding KG1 cells pretreated for 72 h with SK053. (D) Mean clone size (pixel area) ± SEM (n=3 experiments); ^**P<0.0001 vs. controls, one-way ANOVA with Dunnett post-hoc test.