Figure 2.

Clonal and subclonal heterogeneity and variant read frequency distributions. (A) Fifty percent of mutations (85/168) had a variable read frequency (VRF) <25%, and almost half of them (46/168) had a VRF <10%. These values highlight the importance of detecting variants at very low frequencies in newly diagnosed patients. (B) Distribution of the detected variants in the most frequently mutated genes. The number of patients harboring the mutations is indicated below the name of the gene. DIS3 was the only gene in which a mutation VRF >50% was found.