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. 2018 Dec 4;13(12):e0208067. doi: 10.1371/journal.pone.0208067

Fig 2. OVA-specific cellular immune responses in immunized mice.

Fig 2

C57BL/6 mice (n = 10/group) were injected i.m. on Days 0 and 21 with ovalbumin (20 μg) with or without adjuvant. Splenocytes were harvested on Day 28 and analyzed by IFN-γ ELISpot when stimulated by OVA-specific peptides or protein (Panel A). In addition, the in vivo cytolytic activity of OVA-specific CD8+ cells was assessed by the measurement of CFSE-labeled target cells in the splenocytes collected on Day 28 (Panel B). Grouped data is presented as mean + standard error of mean (SEM). *: p<0.05, **: p<0.01, ***: p<0.001 & ****: p<0.0001 for various groups receiving adjuvanted formulations when compared to unadjuvanted control group by one-way ANOVA followed by Dunnett's multiple comparisons test.