Table 4. Cytokine/Chemokine levels in mice after i.m. injection of OVA vaccine formulations.
| Analyte | None | Unadju-vanted | SLA | SLA/LA | Alum | CpG | Alum/CpG | Poly(I:C) | MPLA | Montanide 720 | Addavax |
|---|---|---|---|---|---|---|---|---|---|---|---|
| IFN-γ | 3 (0) | 4 (0) | 4 (1) | 3 (0) | 4 (0) | 4 (1) | 5 (2) | 4 (0) | 3 (0) | 3 (0) | 3 (0) |
| IL-1β | 6 (1) | 7 (0) | 11 (1) | 15 (1) | 9 (1) | 10 (2) | 22 (6) | 9 (1) | 33 (6) | 8 (1) | 13 (1) |
| IL-2 | 2 (0) | 1 (0) | 2 (0) | 2 (0) | 2 (0) | 2 (0) | 4 (1) | 2 (0) | 2 (0) | 1 (0) | 2 (0) |
| IL-4 | 1 (0) | 1 (0) | 2 (0) | 4 (1) | 1 (0) | 1 (0) | 7 (3) | 1 (0) | 1 (0) | 2 (0) | 6 (1) |
| IL-5 | 5 (2) | 22 (6) | 4 (1) | 16 (4) | 20 (3) | 12 (2) | 50 (34) | 21 (11) | 17 (1) | 154 (38) | 202 (22) |
| IL-6 | 4 (1) | 11 (7) | 289 (148) | 1130 (60) | 123 (44) | 66 (7) | 714 (189) | 55 (16) | 1267 (227) | 201 (68) | 299 (39) |
| IL-10 | 2 (0) | 2 (0) | 2 (0) | 2 (0) | 2 (0) | 2 (0) | 3 (1) | 2 (0) | 2 (0) | 2 (0) | 2 (0) |
| IL-17 | 1 (0) | 2 (0) | 2 (0) | 1 (0) | 2 (0) | 1 (0) | 2 (1) | 1 (0) | 1 (0) | 1 (0) | 1 (0) |
| TNF-α | 2 (0) | 2 (0) | 3 (1) | 3 (0) | 2 (0) | 4 (1) | 3 (1) | 2 (0) | 6 (1) | 1 (0) | 2 (0) |
| G-CSF | 3 (1) | 8 (5) | 160 (60) | 523 (47) | 181 (12) | 76 (5) | 443 (97) | 26 (9) | 1086 (190) | 90 (22) | 145 (21) |
| GM-CSF | 1 (0) | 2 (0) | 29 (11) | 82 (8) | 3 (1) | 2 (0) | 9 (3) | 2 (0) | 7 (1) | 4 (1) | 19 (1) |
| IP-10 | 10 (1) | 46 (10) | 226 (27) | 162 (23) | 47 (2) | 361 (52) | 567 (230) | 1044 (154) | 828 (220) | 24 (8) | 46 (11) |
| KC | 24 (11) | 56 (23) | 990 (304) | 1383 (68) | 299 (35) | 133 (45) | 1282 (561) | 97 (28) | 1253 (85) | 231 (9) | 527 (66) |
| MCP-1 | 40 (22) | 186 (41) | 1621 (321) | 1520 (114) | 342 (22) | 410 (21) | 1082 (498) | 622 (140) | 1112 (48) | 487 (16) | 981 (94) |
| MIP-1α | 4 (1) | 5 (1) | 78 (18) | 56 (8) | 15 (1) | 70 (6) | 147 (53) | 28 (5) | 219 (28) | 58 (1) | 162 (19) |
| MIP-1β | 0 (0) | 4 (3) | 147 (26) | 126 (17) | 23 (5) | 146 (16) | 330 (115) | 89 (7) | 566 (85) | 121 (7) | 336 (44) |
| MIP-2 | 11 (1) | 22 (11) | 491 (125) | 660 (34) | 154 (15) | 105 (43) | 1333 (341) | 94 (58) | 1836 (334) | 438 (27) | 1111 (108) |
C57BL/6 mice (n = 3/group) were injected i.m. with PBS (no vaccine) or vaccine formulations containing OVA (20 μg) with or without adjuvant. The levels of 17 immune-related proteins were assessed in protein extracts derived from T.A. muscles 6 hours following injection. Average levels (pg/mg) per group are shown with standard error of mean (SEM) in brackets. Values underlined in bold indicate the measured levels ranked in the top three for a particular analyte amongst the various groups tested and were at least 2-fold higher than values obtained with unadjuvanted formulation.