Table 1. Conditions for storage of air-dried B. subtilis spores*.
Condition | Material additives | Amount of initial spores | Length of storage | Temperature (°C) | Atmospheric conditions |
---|---|---|---|---|---|
500 year desiccation | n.a. | 106 | 2 years | 20 ± 3 | Ambient air |
-80°C | n.a. | 107 | 360 days | -80 | Ambient air |
Vacuum | n.a. | 107 | 450 days | 20 ± 3 | 10−7 Pa pressure |
Controlled ambient air | n.a. | 107 | 10 years | 4 | 80% N2, 20% O2 |
Anoxic | n.a. | 107 | 10 years | 4 | 99.9% CO2 |
Simulated Mars regolith powder | 47.7% Na-montmorillonite, 9.9% kaolinite, 21.3% hematite, 13.0% anhydrite, 7.1% MgSO4, 1.0% NaCl, 2.5% Na2O, 3.4% MgO, 14.1% Al2O3, 34.6% SiO2, 5.1% SO3, 0.2% Cl-, 0.2% K2O, 6.1% CaO, 0.1% TiO, and 18.5% FeO | 107 | 10 years | 4 | Ambient air |
Halite powder | 99.5% NaCl | 107 | 10 years | 4 | Ambient air |
*Unless otherwise noted, storage was performed in ambient air, 40 + /- 5% relative humidity, and normal laboratory conditions.
n.a. is defined as not-applicable