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. 2018 Dec 4;13(12):e0208425. doi: 10.1371/journal.pone.0208425

Table 1. Conditions for storage of air-dried B. subtilis spores*.

Condition Material additives Amount of initial spores Length of storage Temperature (°C) Atmospheric conditions
500 year desiccation n.a. 106 2 years 20 ± 3 Ambient air
-80°C n.a. 107 360 days -80 Ambient air
Vacuum n.a. 107 450 days 20 ± 3 10−7 Pa pressure
Controlled ambient air n.a. 107 10 years 4 80% N2, 20% O2
Anoxic n.a. 107 10 years 4 99.9% CO2
Simulated Mars regolith powder 47.7% Na-montmorillonite, 9.9% kaolinite, 21.3% hematite, 13.0% anhydrite, 7.1% MgSO4, 1.0% NaCl, 2.5% Na2O, 3.4% MgO, 14.1% Al2O3, 34.6% SiO2, 5.1% SO3, 0.2% Cl-, 0.2% K2O, 6.1% CaO, 0.1% TiO, and 18.5% FeO 107 10 years 4 Ambient air
Halite powder 99.5% NaCl 107 10 years 4 Ambient air

*Unless otherwise noted, storage was performed in ambient air, 40 + /- 5% relative humidity, and normal laboratory conditions.

n.a. is defined as not-applicable