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. 2018 Jan 9;98(4):449–464. doi: 10.1093/biolre/ioy004

Figure 2.

Figure 2.

MED13 knockdown inhibits embryo transcription and cell cycle progression. One-cell stage embryos were microinjected with the indicated morpholino, then cultured to the 2C or 4C stage. (A) EU staining as an indicator of transcription levels. (B) Quantification of EU staining. N = 3, total of 23–24 embryos/group; *P ≤ 0.05, T-test. (C) EdU staining as an indicator of DNA replication. (D) Quantification of EdU staining. N = 3, total of 20–24 embryos/group; *P ≤ 0.05, T-test. (E) Gamma H2AX staining as an indicator of DNA double strand breaks. (F) Quantification of γH2AX staining. N = 4, total of 14–36 embryos/group; *P ≤ 0.05, T-test. (G) Immunoblot analysis of cyclin B1 in late 2C-stage embryos (upper panel). Actin was used as a loading control (lower panel). Numbers indicate apparent Mr × 10−3. Lysate of 20 embryos in each lane. Blot is representative of N = 3 independent replicates. All graphs show mean ± s.e.m. All bars = 20 μm.