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. 2018 Jan 9;98(4):449–464. doi: 10.1093/biolre/ioy004

Figure 3.

Figure 3.

MED13 knockdown impairs transcription of genes required for the OET, including cell cycle and chromatin-remodeling proteins. One-cell stage embryos were microinjected with the indicated morpholino, then cultured to the late 2C stage. RNA sequencing was performed on independent pools of 40 embryos per group. (A) Unsupervised hierarchical clustering of gene expression differences in Scr-MO (Scr)- and Med13-MO (M13)-injected embryos. (B) Numbers of altered transcripts of the indicated types in Med13-MO-injected embryos relative to Scr-MO-injected embryos. (C) Highly significant GO biological categories of genes downregulated in Med13-knockdown embryos. Numbers to right of bars indicate number of genes in category. (D) Selected GO cell cycle genes significantly altered in Med13-knockdown embryos. (E) Selected GO chromatin modification genes significantly altered in Med13-knockdown embryos. (F) Percentage of embryos to reach the indicated preimplantation embryo stages following microinjection at the 1C stage with the either Scr-MO, Med13-MO, or Med13-MO and both Smarca4 and Smarcc1 mRNAs. N = 3, 8–12 1C embryos/group for each replicate; *P ≤ 0.05 compared to Scr-MO control at the same time point, #P ≤ 0.05 compared to MED13-MO at the same time point, ANOVA with Holm-Sidak multiple comparisons test. Graph shows mean ± s.e.m. (G) Graph showing total number of chromosomal regions either up- or downregulated for each of the indicated repetitive element classes.