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. 2018 Mar 20;114(8):1098–1114. doi: 10.1093/cvr/cvy069

Figure 2.

Figure 2

  Localization of NkxCE-GFP+ MICs after MI. (A) MI was induced by LAD-ligation in NkxCE-GFP mice (n = 7). After 1 week, hearts were explanted, fixed, cryo-sectioned and subjected to immunoflusorescent or histological staining (IHC-Movat’s). (B) Sections were stained for GFP (green) (DAPI = nuclei, blue). Adjacent sections were stained with Movat’s pentachrome (yellow: collagen fibers; red: muscle). Left upper panels: GFP+ MICs were observed in the infarct area and border zone overlapping with Movat’s yellow stained areas. Right upper panels: in remote myocardium no GFP+ MICs were detected. Lower panels: Stainings for GFP (green) and αActinin (cardiomyocytes, magenta) (DAPI = nuclei, blue). (C) One week after MI was induced in NkxCE-GFP mice (n = 4), hearts were explanted and analyzed in situ under a 2-photon-microscope for GFP+ MICs. Then, hearts were fixed, cryo-sectioned and subjected to IF. (D) Left panel shows the results of 2-photon-microscopy. In situ GFP+ cells (green) are visible in the infarct area and border zone. No GFP+ MICs were detected in the remote myocardium. Right panel: IF confirmed the results of 2-photon-microscopy.