Figure 1.

Global transcriptome analysis segregates individual embryos to their respective stages. (A) Clustering of samples by global transcript levels following PCA. Circles and arrows indicate clustering of samples within broad stages of preimplantation development: oocyte, pre-EGA embryo, and post-EGA embryo. The post-EGA embryos divided into two groups: the 8-cell samples and the morula/blastocyst samples. (B) Hierarchical clustering of sample relatedness based on global transcript levels. Distances of clustering reflect those seen in the PCA graph, with the oocyte stages indicated in purple, the pre-EGA embryo stages in blue, and the post-EGA stages in green.