Figure 7.

The majority of key TFs identified were associated with the 8-cell stage. (A) An increased abundance of regulatory factors was observed at EGA (4C-8C and 8C-MO transitions); many of these TFs have been implicated in early embryonic development and EGA. (B) Normalized expression of upregulated DEGs (4C-8C and 8C-MO pairwise comparisons; padj < 0.05 and FC > 8) containing the TFBS of key regulatory factors within their promoters. (C) Key regulatory factors of macaque EGA are shown in the context of the regulatory network established for human embryonic stem cells (Homo Sapiens v1.2 cell type hESCT0). Interactome includes interactions between key TFs (blue) and targets (green if regulated by multiple key TFs; grey if regulated by one). Network was generated at www.regulatorynetworks.org [62,63].