Skip to main content
. 2015 Aug 4;108(1):148–158. doi: 10.1093/cvr/cvv211

Figure 6.

Figure 6

CARD9-KO inhibited the neointima formation of vein grafts. (A) SMC migration was assessed with 8 µm pore size transwell filters. Representative images are SMC migration response to medium derived from WT and CARD9-KO BMDMs stimulated with necrotic SMCs for 20 h and quantification. Data represent the mean ± SEM; three independent experiments were performed. (B) SMC proliferation response to the conditional medium was assessed by staining of BrdU. Data represent the mean ± SEM; three independent experiments were performed. (C) Growth factors released into culture supernatants from necrotic SMC-treated WT and CARD9-KO BMDMs were determined. Data represent the mean ± SEM; five independent experiments were performed. (D) Staining of PCNA in vein grafts from WT mice and CARD9-KO mice at 4 weeks; n = 6 per group. (E) Staining of H&E and α-SMA in vein grafts from WT mice and CARD9-KO mice at 4 weeks; n = 6 per group. (F) Quantification of the area of lumen and neointima and the ratio of lumen to neointima area. Data represent the mean ± SEM; n = 6 per group. Scale bar: 50 µm. *P < 0.05, **P < 0.01.